Components:1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)